Bütler, Elija Nathanael (2024). Effects of an endoscopic luminal fat challenge and low-carbohydrate high-fat diet on the pancreatic polypeptide family in healthy, lean individuals. (Thesis). Universität Bern, Bern

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Abstract

Introduction: The pancreatic polypeptide (PP) family consists of three closely related polypeptides: neuropeptide Y (NPY), PP and peptide YY (PYY). Despite many structural similarities, PP family members have differing biological effects at various levels of the gastrointestinal tract and nervous system. This study investigates the physiological response of the PP family and its fragments using microliquid chromatography tandem mass spectrometry to firstly, an endoscopic luminal lipid bolus as well as secondly, to a three-day low-carbohydrate high-fat diet (LCHFD) in sedated individuals. Methods: Ten (seven female and three male) healthy, lean volunteers, with a mean age of 26 (sd = 4.4) years and body mass index of 21.2 kg/m2 (sd = 1.4 kg/m2) underwent an oesophagogastroduodenoscopy, during which a standardised oleic and linoleic acid emulsion was placed in the gastric antrum (30 mL) and the post-pyloric duodenal bulb (30 mL). Plasma samples were collected after overnight fasting and 10, 30, 60, 120 and 180 min after an endoscopic luminal lipid bolus. The procedure was repeated after a three-day isocaloric LCHFD. Results: All PP family members displayed a significant increase in response to an endoscopic luminal lipid bolus. Additionally, three days of the LCHFD significantly increase the fasting concentrations of native NPY, PP and their enzymatic cleaved fragments but not PYY. Conclusion: It seems possible that short-term LCHFD within three days can modulate appetite regulation via NPY and/or PP. Thus, it is tempting to speculate that such a short-term diet manoeuvre could already influence appetite regulation via entero-hormonal signalling.

Item Type:	Thesis
Dissertation Type:	Single
Date of Defense:	24 April 2024
Subjects:	600 Technology > 610 Medicine & health
Institute / Center:	04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine
Depositing User:	Sarah Stalder
Date Deposited:	23 Jul 2024 11:05
Last Modified:	25 Jul 2024 03:25
URI:	https://boristheses.unibe.ch/id/eprint/5236

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